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Enzyme Activity Calculator

Compute enzyme activity in U/mL from the rate of absorbance change in a spectrophotometric assay using the Beer-Lambert law. One unit (U) is conventionally the amount of enzyme converting 1 µmol of substrate per minute.

Change in Absorbance (ΔA/min)

Path Length (cm)

Extinction Coefficient (M⁻¹cm⁻¹)

Sample Volume (ml)

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Biology

Protein Concentration Calculator

Compute protein concentration from absorbance at 595 nm using a linear standard curve. The standard back-calculation for Bradford and similar dye-binding assays in molecular biology labs.

Absorbance at 595nm (A595)

Standard Curve Slope

Y-intercept

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Key differences

	Enzyme Activity Calculator	Protein Concentration Calculator
Category	Biology	Biology
Inputs required	4	3
Result	Enzyme Activity (U/ml)	Protein Concentration (μg/ml)
What it does	Compute enzyme activity in U/mL from the rate of absorbance change in a spectrophotometric assay using the Beer-Lambert law. One unit (U) is conventionally the amount of enzyme converting 1 µmol of substrate per minute.	Compute protein concentration from absorbance at 595 nm using a linear standard curve. The standard back-calculation for Bradford and similar dye-binding assays in molecular biology labs.