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Gene Expression Fold Change Calculator

Quantify how much a gene is up- or down-regulated between two conditions using the 2^(−ΔΔCt) method from qRT-PCR data. Use this when comparing treated vs. control gene expression levels.

ΔΔCt Value

Fill in the required fields to see your result.

Biology

Gene Expression Fold Change Calculator

Determine how much a gene is up- or down-regulated by dividing treatment expression by control expression. Used by researchers analyzing qPCR, RNA-seq, or microarray data to quantify gene activity changes.

Treatment Expression Level (units)

Control Expression Level (units)

Fill in the required fields to see your result.

Key differences

	Gene Expression Fold Change Calculator	Gene Expression Fold Change Calculator
Category	Biology	Biology
Inputs required	1	2
Result	Fold Change (x)	Fold Change (fold)
What it does	Quantify how much a gene is up- or down-regulated between two conditions using the 2^(−ΔΔCt) method from qRT-PCR data. Use this when comparing treated vs. control gene expression levels.	Determine how much a gene is up- or down-regulated by dividing treatment expression by control expression. Used by researchers analyzing qPCR, RNA-seq, or microarray data to quantify gene activity changes.