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Both calculators run independently — change the inputs on either side to compare results.
Biology
PCR Efficiency Calculator
Compute the amplification efficiency of a qPCR reaction from the slope of a standard curve fitting Cq values against the log of template concentration. A slope of −3.32 yields 100% efficiency (true doubling per cycle).
Fill in the required fields to see your result.
Biology
PCR Primer Tm Calculator
Calculates the melting temperature (Tm) of short PCR primers using the Wallace rule base-counting method. Use it when designing primers under 20 bases or for a quick sanity check before ordering oligos.
Fill in the required fields to see your result.
Key differences
| PCR Efficiency Calculator | PCR Primer Tm Calculator | |
|---|---|---|
| Category | Biology | Biology |
| Inputs required | 1 | 4 |
| Result | PCR Efficiency (%) | Melting Temperature (°C) |
| What it does | Compute the amplification efficiency of a qPCR reaction from the slope of a standard curve fitting Cq values against the log of template concentration. A slope of −3.32 yields 100% efficiency (true doubling per cycle). | Calculates the melting temperature (Tm) of short PCR primers using the Wallace rule base-counting method. Use it when designing primers under 20 bases or for a quick sanity check before ordering oligos. |