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Both calculators run independently — change the inputs on either side to compare results.

Biology

PCR Primer Tm Calculator

Calculates the melting temperature (Tm) of short PCR primers using the Wallace rule base-counting method. Use it when designing primers under 20 bases or for a quick sanity check before ordering oligos.

Fill in the required fields to see your result.
Biology

Cell Density Calculator

Estimate cell concentration in cells/mL from a haemocytometer count using cellCount, the number of squares counted, and the dilution factor. Returns cells per millilitre.

Fill in the required fields to see your result.

Key differences

PCR Primer Tm CalculatorCell Density Calculator
CategoryBiologyBiology
Inputs required43
ResultMelting Temperature (°C)Cell Density (cells/ml)
What it doesCalculates the melting temperature (Tm) of short PCR primers using the Wallace rule base-counting method. Use it when designing primers under 20 bases or for a quick sanity check before ordering oligos.Estimate cell concentration in cells/mL from a haemocytometer count using cellCount, the number of squares counted, and the dilution factor. Returns cells per millilitre.