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Both calculators run independently — change the inputs on either side to compare results.
Biology
PCR Primer Tm Calculator
Calculates the melting temperature (Tm) of short PCR primers using the Wallace rule base-counting method. Use it when designing primers under 20 bases or for a quick sanity check before ordering oligos.
Fill in the required fields to see your result.
Biology
PCR Efficiency Calculator
Compute the amplification efficiency of a qPCR reaction from the slope of a standard curve fitting Cq values against the log of template concentration. A slope of −3.32 yields 100% efficiency (true doubling per cycle).
Fill in the required fields to see your result.
Key differences
| PCR Primer Tm Calculator | PCR Efficiency Calculator | |
|---|---|---|
| Category | Biology | Biology |
| Inputs required | 4 | 1 |
| Result | Melting Temperature (°C) | PCR Efficiency (%) |
| What it does | Calculates the melting temperature (Tm) of short PCR primers using the Wallace rule base-counting method. Use it when designing primers under 20 bases or for a quick sanity check before ordering oligos. | Compute the amplification efficiency of a qPCR reaction from the slope of a standard curve fitting Cq values against the log of template concentration. A slope of −3.32 yields 100% efficiency (true doubling per cycle). |