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Primer Melting Temperature Calculator

Estimate the melting temperature (Tm) of a PCR primer from its GC content and length using the simplified Wallace-rule approximation. Useful for quickly designing and checking primers before running PCR.

GC Content (%)

Primer Length (bp)

Fill in the required fields to see your result.

Biology

Hardy-Weinberg Equilibrium Calculator

Verify the Hardy-Weinberg equilibrium identity p² + 2pq + q² = 1 from two allele frequencies. Foundational population-genetics check that, in the absence of evolutionary forces, allele and genotype frequencies remain constant across generations.

Frequency of allele p

Frequency of allele q

Fill in the required fields to see your result.

Key differences

	Primer Melting Temperature Calculator	Hardy-Weinberg Equilibrium Calculator
Category	Biology	Biology
Inputs required	2	2
Result	Melting Temperature (°C)	Total Genotype Frequency
What it does	Estimate the melting temperature (Tm) of a PCR primer from its GC content and length using the simplified Wallace-rule approximation. Useful for quickly designing and checking primers before running PCR.	Verify the Hardy-Weinberg equilibrium identity p² + 2pq + q² = 1 from two allele frequencies. Foundational population-genetics check that, in the absence of evolutionary forces, allele and genotype frequencies remain constant across generations.