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Both calculators run independently — change the inputs on either side to compare results.

Protein Concentration Calculator

Compute protein concentration from absorbance at 595 nm using a linear standard curve. The standard back-calculation for Bradford and similar dye-binding assays in molecular biology labs.

Absorbance at 595nm (A595)

Standard Curve Slope

Y-intercept

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Biology

Enzyme Activity Calculator

Compute enzyme activity in U/mL from the rate of absorbance change in a spectrophotometric assay using the Beer-Lambert law. One unit (U) is conventionally the amount of enzyme converting 1 µmol of substrate per minute.

Change in Absorbance (ΔA/min)

Path Length (cm)

Extinction Coefficient (M⁻¹cm⁻¹)

Sample Volume (ml)

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Key differences

	Protein Concentration Calculator	Enzyme Activity Calculator
Category	Biology	Biology
Inputs required	3	4
Result	Protein Concentration (μg/ml)	Enzyme Activity (U/ml)
What it does	Compute protein concentration from absorbance at 595 nm using a linear standard curve. The standard back-calculation for Bradford and similar dye-binding assays in molecular biology labs.	Compute enzyme activity in U/mL from the rate of absorbance change in a spectrophotometric assay using the Beer-Lambert law. One unit (U) is conventionally the amount of enzyme converting 1 µmol of substrate per minute.