Enzyme Activity Calculator vs Protein Concentration Calculator
Two calculators for the same neighbourhood of questions. Here is what each measures, what you feed in, and where you'd reach for one over the other.
Enzyme Activity Calculator
Compute enzyme activity in U/mL from the rate of absorbance change in a spectrophotometric assay using the Beer-Lambert law. One unit (U) is conventionally the amount of enzyme converting 1 µmol of substrate per minute.
- Answers
- Enzyme Activity (U/ml)
- Inputs
- Change in Absorbance, Path Length, Extinction Coefficient, Sample Volume
Protein Concentration Calculator
Compute protein concentration from absorbance at 595 nm using a linear standard curve. The standard back-calculation for Bradford and similar dye-binding assays in molecular biology labs.
- Answers
- Protein Concentration (μg/ml)
- Inputs
- Absorbance at 595nm, Standard Curve Slope, Y-intercept
At a glance
| Enzyme Activity Calculator | Protein Concentration Calculator | |
|---|---|---|
| Category | Biology | Biology |
| Answers | Enzyme Activity | Protein Concentration |
| Inputs | 4 | 3 |
| Result unit | U/ml | μg/ml |
When to use each
Compute enzyme activity in U/mL from the rate of absorbance change in a spectrophotometric assay using the Beer-Lambert law. One unit (U) is conventionally the amount of enzyme converting 1 µmol of substrate per minute.
Compute protein concentration from absorbance at 595 nm using a linear standard curve. The standard back-calculation for Bradford and similar dye-binding assays in molecular biology labs.